Principles of Fluorescence Spectroscopy

Abstract

The first edition of Principles was published in 1983, and the second edition 16 years later in 1999. At that time I thought the third edition would not be written until 2010 or later. However, the technology of fluorescence has advanced at an accelerating pace. Single-molecule detection and fluorescence-correlation spectroscopy are becoming almost routine. New classes of probes have appeared, such as the semiconductor nanoparticles, or QDots, and genetically engineered green fluorescent probes. Additionally, it is now becoming possible to control the excited states of fluorophores, rather than relying only on spontaneous emission. These developments are changing the paradigm of fluorescence, from a reliance on organic fluorophores, to the use of genetic engineering, nanotechnology, and near-field optics. I wish to express my appreciation and special thanks to the individuals who have assisted me in the preparation of the book. These include Ignacy Gryczynski for assistance with the figures, Krystyna Gryczynski for drawing the figures, Joanna Malicka for proofreading the chapters, Kazik Nowaczyk for the cover design and color digitizing of all figures, Tim Oliver for typesetting, and the NIH for their support of my laboratory. And finally, Mary, for her endless hours of typing, correspondence and support